Novel inter-protein cross-link identified in the GGH-ecotin D137Y dimer
نویسندگان
چکیده
منابع مشابه
Determining protein-protein interactions by oxidative cross-linking of a glycine-glycine-histidine fusion protein.
The Ni(II) complex of the tripeptide NH2-glycine-glycine-histidine-COOH (GGH) mediates efficient protein-protein cross-linking in the presence of oxidants such as oxone and monoperoxyphthalic acid (MMPP). Here we demonstrate that GGH fused to the amino terminus of a protein can still support cross-linking. The tripeptide was expressed at the amino terminus of ecotin, a dimeric macromolecular se...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2009
ISSN: 0961-8368
DOI: 10.1110/ps.ps.46601